Banca de DEFESA: ROBERT YONEL MÁRQUEZ GUTIERREZ
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE: ROBERT YONEL MÁRQUEZ GUTIERREZ
DATA: 09/02/2022
HORA: 09:00
LOCAL: remoto
TÍTULO:
Hop (Humulus lupulus L.) development in subtropical regions and genome wide analyses of MADS-box gene family |
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PALAVRAS-CHAVES:
development, Humulus lupulus, MADS-box genes, phenology |
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PÁGINAS: 76
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:
Plants have evolved to time their leafing, flowering and fruiting in appropriate seasons for growth, reproduction and resting. These processes vary considerably between plants from temperate, tropical and subtropical regions. With the aim to establish the hop culture in the southeast of Brazil, a subtropical region, it becomes indispensable to know the pattern of development of this plant in this region. This involves knowing the endogenous and exogenous factors that dictate the phase transition. Here we performed two works with the aim to know the pattern of development of hop in this subtropical region. In the first work, we study the phenology of this plant, focusing on the flowering as a critical phase. Here we analyzed the forms that adopt the plants and the expression pattern of microRNAs 156/172 during the development. The morphologic parameters (number of nodes, heteroblasty, flowers), diminution of miR156 levels, and increase of miR172 levels, suggested that flowering of hop in subtropical regions is dependent on the age of plants and can occur any time of year. Notwithstanding, the months between October and November, is ideally considering the water disponibility by rain. In the second work, we analyzed in silico the MADS box family, and found 65 genes in the hop genome. The phylogenetic analysis classified them in 11 subfamilies within MIKCc group and in three subfamilies with respect to type I group. In the group MIKCC, we found that the subfamilies FLC and AGL17 are absent in the hop genome, both involved as flowering repressor and activator, respectively. In the case of FLC, these results demonstrate that hop does not precise vernalization to flower. Two members of the AGL15 subfamily could participate in flowering transition in hop plants. The presence of A-class, B-class, C/D-class, and E-class genes, together with an expression profile, permitted us to hypothesize the dynamic of expression of these genes and how this could influence floral architecture. The same expression profile permitted us to identify a gene expressed exclusively in the lupulin glands, and according to the gene ontology term, this gene could participate as a positive regulator in the expression of genes involved in the secondary metabolism in the lupulin glands. |
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MEMBROS DA BANCA:
Externo à Instituição - VAGNER AUGUSTO BENEDITO - WVU (Membro)
Interno - PAULO EDUARDO RIBEIRO MARCHIORI (Suplente)
Externo à Instituição - LEO RUFATO - UDESC (Membro)
Presidente - ANTONIO CHALFUN JUNIOR (Membro)
Externo à Instituição - ANDRÉ ALMEIDA LIMA - FIOCRUZ-MG (Suplente)