Banca de DEFESA: JÚLIA DE CARVALHO COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE: JÚLIA DE CARVALHO COSTA
DATA: 11/02/2026
HORA: 08:00
LOCAL: Anfiteatro da Fisiologia Vegetal/ Online Google Meet
TÍTULO:

CHARACTERIZATION OF THE SPL GENE FAMILY AND ETHYLENE-MEDIATED REGULATION OF THE MIR156/MIR172/FTL/FT NETWORK IN HOPS (Humulus lupulus)

PALAVRAS-CHAVES:

Humulus lupulus, SPL, miR156, SBP-box, EREs.

PÁGINAS: 50
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

SPL (Squamosa Promoter-Binding Protein-Like) transcription factors belong to an essential multigene family that coordinates crucial biological processes, including the juvenile-to-adult phase transition and floral development in plants. Hops (Humulus lupulus L.) are a high-value commercial crop due to the secondary metabolites accumulated in their female inflorescences (cones), however, the regulatory mechanisms governing the development of these organs and the phase transition remain poorly understood. MicroRNAs (miRNAs) are small non-coding RNAs that regulate post-transcriptional gene expression and are the main modulators of the SPL pathway. In the present study, through genomic identification and miRNA target prediction, we identified 17 HlSPL genes in the hop genome and characterized hlu-miR156 as their primary putative regulator. Phylogenetic analysis revealed that the HlSPL genes are distributed across nine clades, showing a close orthologous relationship with Cannabis sativa. Molecular characterization confirmed the presence of the conserved SBP domain and ethylene response elements (EREs) in their promoter regions, suggesting an integration between age signals and ethylene signaling. To validate the role of these genes, we analyzed their expression profile by RNA-seq in different tissues and cone developmental stages. Our results revealed tissue-specific expression patterns, with coordinated regulation during inflorescence maturation and significant repression in specialized lupulin glands. These data indicate that the miR156-HlSPL module acts as a central regulatory hub in hops, controlling plant architecture and floral identity. This work provides a solid molecular basis for genetic improvement and biotechnology strategies aimed at optimizing hop productivity and adaptation in new cultivation frontiers.

MEMBROS DA BANCA:
Externo à Instituição - VAGNER AUGUSTO BENEDITO - UMES (Membro)
Externo à Instituição - SOLANGE APARECIDA SÁGIO - UFT (Suplente)
Interno - JOYCE PEREIRA ALVARENGA - UFLA (Suplente)
Externo ao Programa - EVANDRO NOVAES - DBI/ICN (Membro)
Presidente - ANTONIO CHALFUN JUNIOR (Membro)