Banca de QUALIFICAÇÃO: DANYLLO AMARAL DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: DANYLLO AMARAL DE OLIVEIRA
DATA: 08/02/2023
HORA: 14:00
LOCAL: remoto
TÍTULO:

SNPs can correctly separate Eucalyptus species and identify the genomic composition of interspecific hybrids

PALAVRAS-CHAVES:

Silviculture, Molecular markers, Population structure, Hybridization

PÁGINAS: 20
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

Eucalyptus is an economically important genus comprising more than 700 species in different sections according to Brooker (2000). The necessity to increase the production and combine different characteristics in the breeding programs makes the producers use hybridization strategy. However, the species identification by morphological characteristics is a challenge. In this study we evaluated SNPs markers ability to correctly identify species, provenances, and some hybrids using 485 eucalyptus trees from 18 different species and 44 hybrids. SNP genotyping was done using chip Axiom Array previously developed to probe 72K SNPs in Eucalyptus and Corymbia. After the filtering analysis, the dataset contained 22,230 SNPs genotyped in 528 samples. Structure analysis was done using the SNPs data with FastStructure. StructureSelector indicated K=20 as the best clustering model for the data. This model could correctly separate all Eucalyptus sections, assigned each of the 18 species to a different cluster (except E. pilularis and E. cloeziana) but was unable to distinguish provenances within species. Despite that, when we analyzed dataset from individual sections and species, we could have some provenances separated. Regarding the hybrids, there was the presence of unexpected species at the crossing when we used FastStructure K=20 to analyze the hybrid’s pedigree. On average Faststructure could correctly predict approximately 70% of the hybrids’ composition. When we evaluated all 44 hybrids: 28 hybrids had all the species registered in the pedigree correctly identified in their estimated composition, 43 had at least 50% and only one had no parental species correctly identified. A supervised analysis using the hibrids’ known species was done using Admixture software. Results were similar to that of FastStructre. Thus, SNPs markers were able to correctly and effectively discriminate species, sections and some provenances of the Eucalyptus genus according Brooker’s classification, in addition to being able to determine the hybrid composition with relatively high accuracy.

MEMBROS DA BANCA:
Externo à Instituição - DARIO GRATTAPAGLIA - EMBRAPA (Membro)
Presidente - EVANDRO NOVAES (Membro)
Interno - FLAVIA MARIA AVELAR GONCALVES (Membro)
Externo à Instituição - PAULO HENRIQUE MULLER DA SILVA - IPEF (Membro)
Interno - VINICIUS QUINTAO CARNEIRO (Suplente)