Banca de DEFESA: GUILHERME TOMAZ BRAZ

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE: GUILHERME TOMAZ BRAZ
DATA: 13/07/2018
HORA: 08:00
LOCAL: Departamento de Biologia - Anfiteatro DBI02
TÍTULO:

Chromosome identification of corn (Zea mays mays L.) and its relatives using oligo-based FISH barcod

PALAVRAS-CHAVES:

FISH, oligonucleotide, oligo-probes, maize, karyotype, pachytene

PÁGINAS: 15
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Vegetal
RESUMO:

The accurate chromosome identification is the most important fundament for the success on
cytogenetics research. Different strategies based on conventional and molecular cytogenetics
analyses have been used to create chromosome-specific marks to unambiguously distinguish
homologous chromosomes pairs from each other. A variable fluorescent in situ hybridization
probes were generated based on large genomic insertions, single DNA repeats and single copy
genes. Even though these strategies are useful, they are time-consuming, labor-intensive and
have low repeatability across different laboratories. Oligonucleotides-based FISH probes,
recently developed, showed to be a repeatable, cheap and easy methodology for chromosome
identification. In our current study, we showed that a barcode system based on oligo-FISH
probes can be used to identify all 20 chromosomes of corn in metaphase chromosome spreads
and meiotic pachytene stage. The probes designed for corn generated a similar FISH signals
pattern in its relatives chromosomes, showing this is a powerful method for evolutionary studies.
We discussed about some karyotype variations related with knobs length and distributions, and a
paracentric inversion of chromosome 4 of Zea luxurians.

MEMBROS DA BANCA:
Presidente - 1252324 - GIOVANA AUGUSTA TORRES
Interno - 1717914 - VANIA HELENA TECHIO
Externo à Instituição - ELIANA REGINA FORNI MARTINS - UNICAMP
Externo à Instituição - LYDERSON FACIO VICCINI - UFJF
Externo à Instituição - MAGDALENA VAIO - URep
Externo à Instituição - LUDMILA CRISTINA OLIVEIRA - UFLA
Externo à Instituição - LAIANE CORSINI ROCHA - UFLA