Lethal and sublethal effects of isocycloseram on Myzus persicae and its natural predator Allograpta exotica
Pollinator, Side effects, Prey, Integrated pest management, Sustainable agriculture
Insect pests, including green peach aphids, Myzus persicae, cause a lot of damage to different crops, and the use of insecticides is a frequent tool for their control. The hoverfly insectivore larvae are natural predators of aphids and other plant-sucking insects and play a critical role in regulating pest populations in agriculture. However, the use of insecticides can have lethal or even sublethal effects on these beneficial insects, essentially affecting their biological life traits and their effectiveness in controlling aphids. This study aimed to evaluate the Lethal and sublethal effects of isocycloceram on the longevity and reproductive traits of M. persicae and its natural enemy, Allograpta exotica on the biological traits: longevity, fecundity, pupal weight, developmental time and predation rate. The study was carried out at the Molecular Entomology and Ecotoxicology laboratory (MEET) at the Federal University of Lavras. The creation of M. persicae was maintained under controlled temperature conditions 20 ± 2°C, 70±10% relative humidity and 16-hour photoperiod; however, A. exotica were kept in a climatic room 25 ± 2°C and 50±7% relative humidity, and the photoperiod was maintained at 12:12 hours. The toxicological bioassays consisted of exposing the adult M. persicae and A. exotica larvae to seven doses each one to determine the LC50 and LC90. The mortalities caused by the isocycloceram were evaluated after 48 hours, and the dose-response cruve was determined. Subsequently, the sublethal effects on the adult M. persicae and A. exotica larvae were assessed. Fifty replicates of 50 adults of M. persicae and three replicates of 50 larvae of A. exotica were exposed to the sublethal concentrations LC1, LC5, LC10, LC20, and the control. The Longevity and fecundity of M. persicae were assessed each day until death. The evaluation of A. exotica larvae began from the 2nd instar to the death of the new generation adult. Each larva was offered 20 aphid adults to assess their development time, predatory capacity, and the biological traits of adults emerging from sublethal exposed larvae were investigated. The results showed the difference between all parameters evaluated. Thus, according to the results, the use of isocycloceram in Integrated Pest Management (IPM) programs requires further research to ensure the safety of natural enemies.