Biochemical treatments with potential effect on Plasmopara viticola
Enraizamento;Controlebiológico;Trichoderma;Bacillus
Grapevine production is strongly constrained by downy mildew caused by Plasmopara viticola, considered one of the most important diseases affecting vineyards worldwide. The management of this disease relies mainly on the frequent application of synthetic fungicides, which increases production costs, environmental risks, and the selection of resistant pathogen populations. In this context, the search for sustainable control alternatives has increased interest in the use of bioactive compounds produced by microorganisms and plants. Rhamnolipid biosurfactants produced by Pseudomonas spp. have demonstrated antimicrobial activity against different phytopathogens, especially oomycetes, while plant-derived proteases have the potential to degrade structural proteins associated with pathogen development and infection. Thus, the objective of this project is to evaluate the effects of rhamnolipids produced by Pseudomonas spp. and plant-derived proteases on infective structures of P. viticola, as well as their capacity to reduce downy mildew severity in Vitis vinifera var. Syrah. Initially, Pseudomonas isolates will be obtained from soil and water samples and screened for biosurfactant production through oil displacement and emulsification index assays. Crude extracts and purified mono- and di-rhamnolipid fractions will be evaluated for their effects on sporangia, zoospore release, and zoospore motility of P. viticola. In parallel, different concentrations of plant-derived proteases will be tested against the same pathogen structures. The most promising treatments will subsequently be evaluated in detached leaf assays and field experiments to determine their capacity to reduce disease severity. The results of this project are expected to contribute to the generation of scientific information regarding the potential use of bacterial biosurfactants and plant-derived proteases as complementary tools for the sustainable management of grapevine downy mildew.