GUT MICROBIAL COMMUNITY STRUCTURE AND ITS FUNCTIONAL ROLE IN RESISTANCE IN Musca domestica
Gut microbes; Larval bioassays; Molecular entomology; Resistance modulation; Dipterans.
Insecticide resistance in dipteran insects has become an increasingly major concern for agricultural productivity, environmental sustainability, and public health. Although, resistance has commonly been attributed to genetic mutations and biochemical detoxification mechanisms within the target insect, there has been growing evidence suggesting that microorganisms inhabiting the insect gut may influence how insects respond to chemical stressors such as xenobiotics, including chemical insecticides and plant-based biopesticides . This work plan seeks to examine the role of gut microbiota in shaping insecticide resistance in different stages of the house fly Musca domestica. Larvae of this selected dipteran specie will be exposed to standardized insecticide bioassays to determine baseline susceptibility patterns, after which gut microbial communities will be experimentally altered through antibiotic treatment. Changes in gut microbiota composition will be assessed using high-throughput 16S rRNA gene sequencing, and microbial profiles will be related to larval mortality and sublethal responses following insecticide exposure. By combining toxicological assays with microbial community analysis, this study aims to identify microbial groups potentially involved in modifying insecticide resistance, either through direct interaction with toxic compounds or through effects on host physiological processes. The outcomes of this research are expected to advance understanding of insect–microbe interactions in dipteran larvae and provide a basis for more integrative approaches to insecticide resistance management