Banca de DEFESA: GABRIEL LASMAR DOS REIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE: GABRIEL LASMAR DOS REIS
DATA: 20/02/2020
HORA: 09:00
LOCAL: Anfiteatro Setor de Fisiologia Vegetal – Departamento de Biologia – UFLA
TÍTULO:

microRNAs related with Anthocyanin Biosynthesis pathway in tomato

PALAVRAS-CHAVES:

Tomato; Anthocyanin Biosynthesis; microRNAs; CRISPR-Cas9.

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

The anthocyanins are compounds from the secondary metabolism of plants and belong to the group of flavonoids. They are responsible to the blue, red and purple pigmentation in many plant tissues, as leaves, flowers, seeds and fruits. For plants they are mainly responsible to the growth and development, defense against pathogens, drought tolerance and protection against UV radiation. Besides, the anthocyanin are extremely benefit to human health, they fight and prevent a range of chronic diseases. Among these benefits, antioxidant effects, prevention against cardiovascular diseases and cancer stand out. The principal vegetables and fruits present in our diet accumulate anthocyanin just in small quantities, and in most cases being restricted to the epidermal layers of the edible parts. Since the peel usually represents less than 5% of the total mass a high anthocyanin accumulation is desirable, and the tomato is a great candidate for this improvement, since it is the principal vegetable in worldwide. MicroRNAs (miRNAs) are a class of small non-coding RNAs that contain 21-24 nucleotides and regulate gene expression post transcriptionally, by mRNA cleavage or suppressing translation. In tomato, the miRNAs role in anthocyanin biosynthesis pathway is still not well characterized. Aiming for a better understand of the actuation of the miRNAs in this pathway, we performed the prediction, validation and knockout of some miRNAs as well as the expression analyzes for some of the anthocyanin pathway related genes. The miRNA target prediction identified the genes Anthocyanidin synthase (ANS), SlAN1 and Chalcone Synthase (CHS) being possible regulate by the miR5368, miR6024 and miR9471respectively. We analyzed the expression of these miRNAs and anthocyanin related genes in Micro-Tom triple mutant Aft/atv/hp2 and Micro-Tom Wild-Type by RT-qPCR. Posteriorly, we performed the knockout of each miRNA in Micro-Tom Wild-Type by CRISPR-Cas9. As a result, we observed transformed explants with a purple phenotype in leaf veins and stem, indicating that the miR5368, miR6024 and miR9471 promote negative regulation in the anthocyanin biosynthesis pathway acting in the genes ANS, SlAN1 and CHS respectively.

MEMBROS DA BANCA:
Presidente - ANTONIO CHALFUN JUNIOR (Membro)
Externo ao Programa - RAPHAEL RICON DE OLIVEIRA - DBI (Suplente)
Externo à Instituição - EDER MARQUES DA SILVA - USP (Membro)
Externo à Instituição - SAMUEL CHAVES SILVA - UFMG (Suplente)
Externo à Instituição - VAGNER AUGUSTO BENEDITO - UFLA (Membro)