Banca de QUALIFICAÇÃO: ISAAC FILIPE MOREIRA KONIG

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: ISAAC FILIPE MOREIRA KONIG
DATA: 24/11/2022
HORA: 08:00
LOCAL: Auditório do Departamento de Medicina e Google Meet: https://meet.google.com/cxi-jaes-pke
TÍTULO:

Comparative toxicological prospection of carvacrol and its acetylated derivative in the zebrafish (Danio rerio) and human blood models   

PALAVRAS-CHAVES:

Zebrafish; human blood; pesticide; carvacrol acetate; toxicity

PÁGINAS: 110
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
RESUMO:

The search for natural-based compounds presenting pesticide activity has intensified in

the last decade. However, the lack of comparative toxicological studies is a limiting

factor for their commercialization. The aim of this study was to compare the toxicity of

carvacrol (natural), acetylcarvacrol (semi-synthetic), and fipronil (synthetic) employing

two models: human blood and developing zebrafish (Danio rerio). A series of

concentrations was used to evaluate the effects of these chemicals on hemolytic activity,

erythrocyte morphology, leucocyte viability, and DNA fragmentation. In the zebrafish

model, we assessed survivability, hatchability, malformations, morphometrics,

behavior, mitochondrial bioenergetics, apoptosis, reactive oxygen species as well as

gene expression. Our findings suggest that fipronil presents relatively high toxicity to

human blood cells compared to the other chemicals. Carvacrol at high concentrations

was also toxic, but its use at low concentrations as well as its acetylated derivative

caused reduced damage to blood cells. In the zebrafish model, fipronil exhibited high

acute toxicity following seven days of continuous exposure. It also affected the

expression of genes related to mitochondrial activity, decreased swim bladder surface

area, induced reactive oxygen species, and hypoactivity in the visual motor response

test. Carvacrol and acetylcarvacrol were considerably less toxic to developing zebrafish

compared to fipronil. Acetylation of carvacrol was also responsible for a reduction in

lethality. Taken together, our results show that carvacrol and acetylcarvacrol are safe

replacements for fipronil due to their reduced toxicity in the two models evaluated.

Moreover, chemical modification of a natural compound such as carvacrol would seem

to be a promising alternative to improve its biological activity and reduce its toxicity to

non-target organisms.

MEMBROS DA BANCA:
Externo ao Programa - CHRYSTIAN ARAUJO PEREIRA - DME/FCS (Membro)
Externo à Instituição - CLAYTON ZAMBELI OLIVEIRA - UFPB (Suplente)
Interno - FILIPPE ELIAS DE FREITAS SOARES (Membro)
Externo ao Programa - GUSTAVO HENRIQUE ANDRADE MACHADO - DME/FCS (Membro)
Externo ao Programa - JULIANA MESQUITA FREIRE - DQI/ICN (Suplente)
Presidente - SILVANA MARCUSSI (Membro)