Banca de QUALIFICAÇÃO: GABRIELA ESTER FERRAZ

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: GABRIELA ESTER FERRAZ
DATA: 30/08/2024
HORA: 08:00
LOCAL: DBI02 e Google Meet https://meet.google.com/rhn-bqbh-wpw?hs=122&authuser=0
TÍTULO:

Genome-wide characterization of invertases in Arabica coffee and your parents reveals putative genes

PALAVRAS-CHAVES:

Coffea, expression profiles, sucrose, glycoside hydrolase, polyploidy

PÁGINAS: 35
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitotecnia
ESPECIALIDADE: Melhoramento Vegetal
RESUMO:

Invertases are involved in essential physiological processes, as they irreversibly degrade sucrose, generating hexoses that play a role in plant growth and development. Few genes have been characterized in the Coffea, and consequently, the relationship of these enzymes in coffee plant development is not well understood. A total of 65 invertase genes were identified, with 28 in C. arabica (CaINV), 18 in C. canephora (CcINV), and 19 in C. eugenioides (CeINV), with polyploidization being the primary cause of the expansion in the number of CaINV. Synteny analysis along with chromosomal mapping showed that the CaINV genes are mainly derived from C. eugenioides. The cis-acting elements indicated the involvement of invertase genes in three main categories: growth and development, responses to biotic and abiotic stress, and hormonal response. Transcriptome analyses showed that, except for the neutral/alkaline invertase gene CaN/AINV1, all invertase genes of C. arabica and C. canephora were expressed at different stages of fruit development, some constitutively and others conditionally.  Although CaN/AINV1 was not expressed in the evaluated tissues, the presence of two GCN4 cis-elements suggests its involvement in specific processes in tissues or stages not analyzed in this study. Our results open many perspectives for future studies in Coffea, especially for the economically significant species C. arabica and C. canephora, which exhibit particularities regarding disease resistance, tolerance to abiotic stresses, and fruit chemical composition.

MEMBROS DA BANCA:
Externo ao Programa - LUCIMARA CRUZ DE SOUZA - DBI/ICN (Membro)
Externo à Instituição - JOYCE PEREIRA ALVARENGA - UFLA (Suplente)
Presidente - FLAVIA MARIA AVELAR GONCALVES (Membro)
Interno - EVANDRO NOVAES (Membro)
Externo ao Programa - ANTONIO CHALFUN JUNIOR - DBI/ICN (Membro)
Externo ao Programa - ANA CARLA MARQUES PINHEIRO - DCA/ESAL (Membro)