Potential of Arabica coffee genotypes for bean quality
Diversity, cup quality, GC-MS, metabolomics, expression profile,
polyploidy.
Research on Coffea arabica aimed at obtaining promising genotypes for grain quality
has advanced in several areas, including genetic improvement, fruit biochemistry, and
the study of genes related to coffee development and quality. In this study, the
phenotypic and genotypic characterization of 14 progenies derived from the Mundo
Novo cultivar revealed high genetic and phenotypic variability, both among and within
progenies. Intracultivar variation was also evident in commercial cultivars, highlighting
this common feature in Arabica coffee. These results underscore the importance of
monitoring genetic purity in breeding programs and seed propagation. In the
biochemical field, analyses of carbohydrates and differentially abundant metabolites
(DAMs) indicated significant variations, particularly in the early perisperm stage, with
greater distinction among cultivars at 30 days after flowering (DS1). Compounds such
as D-ribose and D-fructose showed similarities among some cultivars, while significant
differences were observed in metabolites related to glycolysis and the tricarboxylic acid
cycle, both involved in the formation of precursors for quality-related compounds.
Finally, considering the essential role of sucrose in plants and its relevance as a
precursor of coffee flavor compounds, invertase genes, which encode enzymes
responsible for the irreversible breakdown of sucrose, were investigated. A total of 65
genes were identified in three Coffea species: 28 in C. arabica, and 18 and 19 in
modern representatives of its parental species, C. canephora and C. eugenioides,
respectively. Polyploidization is likely the main cause of the expansion in the number of
invertase genes in C. arabica. Expression analyses revealed both constitutive and
conditional patterns across different fruit development stages, providing new
perspectives for functional studies, especially in economically significant species such
as C. arabica and C. canephora. Therefore, this study aimed to enrich knowledge on
factors influencing the quality of C. arabica fruits, contributing to the development of
more efficient cultivars aligned with global market demands and promoting the value of
the coffee production chain.