Improved Response to Drought Stress in the Rice Expressing Arabidopsis DREB1a
Stress tolerance, Phenotypic plasticity, Biomass accumulation, Chlorophyll content, Grain yield
Arabidopsis thaliana Dehydration Response Element Binding 1a (AtDREB1a) is a master regulator of abiotic stress. The stress-inducible expression of AtDREB1a is considered a preferred strategy for introducing abiotic stress tolerance in crop plants. Accordingly, this study evaluated the drought stress response of a transgenic rice expressing AtDREB1a under the drought-inducible RD29a promoter during reproductive development by withholding water at the booting stage and measuring key agronomic traits in four greenhouse experiments. Upon rewatering, AtDREB1a plants fully recovered from drought stress, while the wild-type (WT) plants showed only partial recovery and displayed wilted and droopy leaves. Compared to WT, AtDREB1a-expressing plants developed higher biomass and grains per panicle that correlated with higher chlorophyll content and spikelet number. Further, under well-watered conditions, AtDREB1a line did not show yield penalty. Overall, this study shows that inducible expression of AtDREB1a in rice improves yield under drought without imposing growth defects under well-watered conditions.