Banca de DEFESA: JOSÉ MANOEL FERREIRA DE LIMA CRUZ

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE: JOSÉ MANOEL FERREIRA DE LIMA CRUZ
DATA: 30/04/2025
HORA: 08:00
LOCAL: Anfiteatro do DFP
TÍTULO:

Characterization of grapevine root and trunk rot disease and the use of Trichoderma to promote plantlet rooting and growth

PALAVRAS-CHAVES:

diagnosis, Fusarium, pathogenicity, Trichoderma, Vitis

PÁGINAS: 62
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitossanidade
ESPECIALIDADE: Fitopatologia
RESUMO:

Grapevine diseases are a concern for grape and fine wine producers due to their economic impact. Among these, 

root and trunk rot, a new disease that has been observed during the cold storage of plantlets stands out. The 

disease reduces the quality of the plantlets and compromises their survival after definitive transplantation to the f

ield. In this context, the development of new technologies applied to the production of grapevine planting material 

emerges as a promising approach to enhance the physiological quality of the grafted material. The use of 

biotechnological tools aimed at improving plantlet establishment can significantly contribute to mitigate losses 

in nurseries, favoring the initial vigor and rooting of the plantlets. The general objective of this research was to 

determine the etiology of root and trunk rot, as well as to test the potential of Trichoderma in the production of 

grapevine planting material. Initial observations indicated that fungi of the genus Fusarium were associated with 

the disease. Fingerprinting analyses by means of BOX-PCR of 86 Fusarium isolates from diseased plants revealed 

45 genetic groups based on 53 polymorphic bands. Sequencing of tef1 gene fragments of 78 Fusarium isolates from

 healthy (n=30) and diseased (n=49) plants revealed 14 species belonging to the complexes F. oxysporum (FOSC), 

F. fujikuroi (FFSC), F. tricinctum (FTSC) and F. solani (FSSC). Pathogenicity tests in the field and greenhouse 

confirmed that 13 Fusarium species(F. casha, F. fredkrugeri, F. inflexum, F. vaughaniae, F. fujikuroi, F. annulatum,

 F. foetens, F. solani, F. landiae, F. triseptatum, F. guttiforme, F. annulatum and F. chongqingense) were able to cause

 root and trunk rot in grapevine plantlets. Comparative analyses of scanning electron microscopy showed that diseased

 plantlets had xylem vessels obstructed with tyloses and gum. Energy dispersive X-ray spectroscopy analyses showed 

differences in the mineral composition of roots of healthy and diseased plantlets. In healthy roots, Ca was distributed 

more uniformly and part of it was concentrated in the epidermis, while in diseased seedlings, it was in the xylem 

vessels. Silicon was not detected in healthy tissues, but was distributed uniformly in diseased tissues. The present 

study provided information that may be used in the management of root and trunk rot disease in grapevine. In a

nother part of the work, the Trichoderma isolate LFM004 was tested in a field experiment to promote rooting and 

growth of grapevine plantlets. It was observed that isolate LFM004 induced an increase in plantlet set of 98 and 55%, 

respectively, in relation to water and hormone treatments. The dry mass of plantlets treated with the Trichoderma 

isolate were 1.5X and 2.2X greater than the treatments with water and hormone, respectively. The isolate LFM004 

was able to produce indoleacetic acid (IAA) and solubilize phosphate, and these characteristics may be related to the 

activity of promoting rooting and growth of grapevine plantlets. The polyphasic characterization of isolate LFM004 

through micromorphological and molecular analyses revealed that it was a new species, for which we proposed the 

name T. harenae sp. nov. The present study contributed to the description and characterization of a new grapevine 

disease, root and trunk rot, and also to the indication of a possible biotechnological solution to improve seedling 

growth and establishment.

MEMBROS DA BANCA:
Externo à Instituição - PHELLIPPE ARTHUR SANTOS MARBACH - UFRB (Suplente)
Externo ao Programa - PEDRO MARANHA PECHE - DAG/ESAL (Membro)
Interno - MARIA ALVES FERREIRA (Suplente)
Interno - LUDWIG HEINRICH PFENNING (Membro)
Presidente - JORGE TEODORO DE SOUZA (Membro)
Externo à Instituição - CLAUDIA RITA DE SOUZA - EPAMIG (Membro)
Externo à Instituição - CARLOS ANTONIO INACIO - UFRRJ (Membro)