Banca de QUALIFICAÇÃO: DIANA CAROLINA CEDIEL DEVIA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: DIANA CAROLINA CEDIEL DEVIA
DATA: 14/03/2025
HORA: 14:00
LOCAL: Online
TÍTULO:

Use of system biology approach to optimize Nitrogen nutrition during early life development of cattle

PALAVRAS-CHAVES:

Fetal programming, Sexual dimorphism, Zebu beef cows.

PÁGINAS: 100
GRANDE ÁREA: Ciências Agrárias
ÁREA: Zootecnia
RESUMO:

The project aims to integrate animal responses and omics data through a systems biology approach to uncover the molecular and biochemical mechanisms underlying maternal physiological changes and skeletal muscle development in progeny in response to nitrogen nutritional optimization. This study combines cutting-edge science (integrating animal responses with a systems biology approach) with an industrially relevant topic (providing solutions to enhance sustainable beef productivity in tropical regions). A total of 36 pregnant Zebu beef cows, averaging 510 kg and four years of age, will be used. From day 180 to 270 of gestation, cows will be individually fed and randomly assigned to three treatments (n = 12 per treatment): 1) negative control = no supplement, 2) positive control = supplement with urea, and 3) alternative treatment = supplement with slow-release nitrogen. During the experimental period, dry matter intake will be measured, and two digestibility trials, each lasting five days, will be conducted (starting on days 228 and 263 of gestation) to determine nutrient digestibility, nitrogen balance, and microbial protein synthesis (assessed via urinary purine derivatives). Blood glucose concentration and amino acid profiles will be quantified on days 225 and 270 of gestation using colorimetric and HPLC methodologies, respectively. On day 268 of gestation, muscle and liver tissue biopsies will be performed on the cows to extract RNA and protein for quantification of molecular markers associated with energy and nitrogen metabolism. The progeny's productive performance will be assessed at birth and throughout the rearing phase. Muscle tissue samples will be collected at 45 and 210 days of age to evaluate muscle biology using systems biology and cell culture approaches. Following a surgical biopsy of the longissimus thoracis muscle at the level of the 12th and 13th ribs, samples will be stored in liquid nitrogen for subsequent proteomic (HPLC-MS/MS) and transcriptomic (RNA-Seq) analyses, as well as cell isolation for in vitro culture. Raw data will be filtered, quantified, and normalized to identify differentially abundant genes and proteins. Enriched biological processes will be determined through omics data integration. Satellite cells will be isolated from pre-digested macerated tissue, and muscle fibers will be separated via centrifugation and filtration. Pax7+ and BrdU+ cells will be quantified to determine mitotically active cells, and RNA will be extracted from satellite cell cultures for myogenic marker analysis using RT-qPCR techniques.

MEMBROS DA BANCA:
Externo ao Programa - TATHYANE RAMALHO SANTOS GIONBELLI - DZO/FZMV (Membro)
Interno - SARAH LAGUNA CONCEICAO MEIRELLES (Membro)
Externo à Instituição - MARCIO DE SOUZA DUARTE - nd (Suplente)
Presidente - MARCIO ANDRE STEFANELLI LARA (Membro)
Externo à Instituição - JAVIER ANDRÉS MORENO MENESES - U.D.C.A (Membro)
Interno - DANIEL RUME CASAGRANDE (Suplente)