Banca de QUALIFICAÇÃO: ANA KARLA DE LIMA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE: ANA KARLA DE LIMA SILVA
DATA: 06/01/2025
HORA: 14:00
LOCAL: DMV08
TÍTULO:

Brucella abortus rough live attenuated vaccines against bovine brucellosis: a systematic review and assessment of Brazilian vaccine candidates

PALAVRAS-CHAVES:

live vaccine, immunogenicity, residual virulence, mutation, sequencing, DIVA vaccine.

PÁGINAS: 70
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
RESUMO:

Brucellosis is a zoonotic disease of significant economic and public health concern worldwide, transmitted by bacteria of the genus Brucellaspp. Vaccination is a cornerstone for controlling the disease, yet current commercially available vaccines have limitations, including adverse side effects, risk of disease in humans and incomplete differentiation between vaccinated and infected animals. This work aims to describe the efficacy of seven Brucella abortus vaccines candidates in two separate chapters. In the first chapter, a systematic review was conducted to investigate the role of lipopolysaccharide (LPS)-encoding genes in the attenuation, virulence, and immunogenicity of Brucella spp., focusing on natural and artificially mutated strains. Following PRISMA guidelines, a comprehensive search across six databases identified 1,418 studies, of which 65 met the inclusion criteria and are currently under analysis. Preliminary findings suggest that genetic mutations in LPS-related genes such as wbkA, wbkB, and wBoA influence bacterial virulence and immunogenicity, offering potential for developing novel attenuated vaccine candidates. Data analysis thus far highlights the need for further genetic and immunological studies to improve brucellosis control strategies by leveraging LPS gene functions for safer and more effective vaccines. In chapter two, an experimental study in three steps was conducted to assess the vaccination potential of seven B. abortus mutants that were isolated from cattle in Brazil and were selected as candidates based on their genetic profiles using whole genome sequencing (WGS). The experimental evaluation of each strain in mice and cattle concentrated on its immunogenicity, residual pathogenicity, and serological cross-reactivity. Our results demonstrate the potential of the mutant LBAB026 as a differentiating infected from vaccinated animals (DIVA)-compatible vaccine, non-rifampicin resistant candidate that satisfy the safety and effectiveness requirements in mice and, potentially for bovine brucellosis reduction initiatives.  Furthermore, it demonstrates the potential of WGS advances the field of brucellosis vaccine research by offering a potent tool for the logical selection of vaccine candidates, ongoing enhancement of current vaccines, and the discovery of new targets.

MEMBROS DA BANCA:
Externo à Instituição - TELMA MARIA ALVES - CEVA (Suplente)
Presidente - ELAINE MARIA SELES DORNELES (Membro)
Externo à Instituição - DIRCÉIA APARECIDA DA COSTA CUSTÓDIO - UFLA (Membro)
Interno - CARINE RODRIGUES PEREIRA (Suplente)
Externo à Instituição - ANDREY PEREIRA LAGE - UFMG (Membro)