Susceptibility to commonly used antiseptics such as dipping and the alternative use of alcoholic extract of propolis in mastitis pathogens and detection of Brucella spp. in samples from dairy cows housed in a compost barn system
bacterial resistance, minimum inhibitory concentrations, brucellosis
Bovine mastitis is defined as inflammation of the mammary gland, caused mainly by the presence of infectious agents. The disease has a complex and multifactorial etiology, with a high prevalence worldwide. It is considered the biggest cause of economic losses for dairy farming, mainly due to the decrease in milk production and quality, milk disposal and expenses with treatments such as medicines and veterinary services. The high prevalence of mastitis contributes to a high and often indiscriminate use of antimicrobial drugs on dairy farms, leading to the emergence of resistant bacteria, which represents a problem for human and animal health. In order to prevent this infection, mastitis-causing pathogens are constantly exposed to disinfectants for teat dipping. The “dipping” technique, in turn, consists of immersing the animals’ teats in antiseptic solutions before (pre-dipping) and after (post-dipping) milking. Studies demonstrate that the frequent use of antiseptics and disinfectants can contribute to bacterial resistance to these compounds and also contribute to the increase in bacterial resistance to antibiotics, due to cross-resistance mechanisms. Another disease of importance for dairy farming due to the related economic losses and also of importance for public health, is bovine brucellosis, a zoonotic disease with worldwide prevalence and occurrence. Infected animals develop reproductive problems such as miscarriage, retained placenta and infertility. Transmission between cattle occurs through contact with fetal membranes or contaminated food and transmission to humans occurs especially through the consumption of contaminated food. In this context, the compost barn, a confined system that promotes animal comfort and welfare, characterized by a shared bed of organic, which may favor the presence and spread of brucellosis, even more when pregnant animals give birth within this environment. Thus, the objective of this dissertation is to evaluate the susceptibility of the main pathogens causing bovine mastitis to antiseptics commonly used as dipping, as well as to evaluate the susceptibility of these pathogens to four different alcoholic extracts of propolis, as a way of evaluating the antimicrobial potential of this compound. It also aims to investigate the presence of brucellosis in dairy cows housed in a compost barn system.