Banca de DEFESA: BARBARA DO CARMO RODRIGUES VIROTE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE: BARBARA DO CARMO RODRIGUES VIROTE
DATA: 25/04/2019
HORA: 09:00
LOCAL: Sala de Reuniões PPGCV
TÍTULO:

INFLUENCE OF DIETARY INDUCED OBESITY ON REPRODUCTIVE PARAMETERS AND PROLE IN ZEBRAFISH

PALAVRAS-CHAVES:

Danio rerio, overfeeding and transgenerational

PÁGINAS: 50
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Obesity is a transgenerational epigenetic metabolic disorder. Animal models of obesity such as zebrafish (Danio rerio) are useful in helping to understand the signaling pathways involved in this condition. In this study we analyzed, in zebrafish, the effect of maternal and paternal obesity on reproduction and offspring (F1). Animals of both sexes were divided into two groups: the control group (C), who received a balanced diet and the obese group induced by overfeeding (DIO). The feeding protocol was performed for eight weeks and the animals were reproduced weekly. To verify the impact of obesity on the parents, we performed histological analysis by morphometry of the visceral and subcutaneous adipose tissue (TA) and measured the hepatosmatic (HSI) and gonadosomatic (IGS) index. The impact of overfeeding on reproduction, spawning, fecundity and fertilization rate was evaluated. In the offspring, mortality, eclosion, edema, lordosis and tail deformities were analyzed. The parents and offspring were also evaluated in the offspring. levels of glucose and triglycerides. As results we verified that obesity induction culminated in hypertrophy of AT and increase of IHS in both sexes of the parents and increase of IGS in females. Obesity did not cause infertility in the zebrafish. However, parental overfeeding culminated with a higher mortality rate, morphological deformities and elevated plasma levels of glucose and triglycerides, with 100% mortality at 60 dpf. We concluded that the induction of obesity through overfeeding had no effect on the reproductive capacity of zebrafish, but it had deleterious effects on offspring with a high incidence of deformities and mortality.

 

MEMBROS DA BANCA:
Presidente - LUIS DAVID SOLIS MURGAS
Externo ao Programa - ANDRE RODRIGUES DA CUNHA BARRETO VIANNA - DMV
Externo à Instituição - EDUARDO MAURÍCIO MENDES DE LIMA - UnB
Externo à Instituição - GILMARA JUNQUEIRA MACHADO PEREIRA - UFLA
Externo à Instituição - MONICA RODRIGUES FERREIRA MACHADO - UFG